Abstract
In this paper, the hydrolysis conditions to extract protein from excess sludge by thermal pretreatment assisted enzymatic were studied. The optimum technological conditions were the sludge moisture content of 95%, thermal pretreatment temperature of 100 °C, heating time of 3h, initial pH of enzymolysis sludge of 11, enzyme dosage of 3000U/g dry sludge, enzymolysis temperature of 55°C and time of 3h. Up to 52.8% of the protein content of the sludge was extracted, and the dehydration performance of the hydrolyzed sludge was 87.46% higher than that of the original sludge. Therefore, thermal pretreatment-assisted enzymolysis can effectively extract protein from excess sludge and improve its dewatering performance.
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