Extraction of FAME from fish waste by using modified soxhlet method

Abstract

The number of fish waste has increased rapidly over the time. Most of discards composed of head, tail and internal organs are considered to be worthless. In this research, fish waste from Pangasius Hypopthalmus (Patin fish) was being used as the raw material to produce fish oil. Therefore, the objective of this research is to evaluate the effect of different ratio solid to solvent (1:1, 1:2, 1:3, 1:4 and 1:5), temperature (70, 75, 80, 85 and 90 °C) and mixing time (1, 2, 3, 4 and 5 hours) on production of fish oil and to analyze the amount of fatty acid methyl ester (FAME) in the fish oil. Sample preparation was being made by choosing solid fat from internal organ of Patin fish. Modified Soxhlet method was used to extract the fish oil from fish waste and ethanol as the solvent. FAME from extracted fish oil was analyze by using Gas Chromatography Mass Spectrometry (GCMS). The result showed that the highest yield of oil being produced was 59.92 ± 0.926 % at the temperature of 85 °C with 1:2 of ratio solid to solvent and 5 hours of mixing time. Besides, the extracted fish oil for sample 3 contained the highest amount of FAME which is 0.4016 mg/g with majority amount of saturated fatty acid.