Abstract
Extracellular polymeric substances (EPS) have many beneficial functions in soils. Accurate quantification of EPS in soils is crucial. Here, five methods were compared for their suitability for extraction of EPS from Ultisols: hot water extractable polysaccharide (HWEP), hot dilute acid extractable polysaccharide (HDAEP), easily extractable glomalin (EEG), sodium sulfide (SS) and cation exchange resin (CER) method. Humic-acid equivalent (HAE) was used as an indicator for extracellular contamination and ATP for quantifying intracellular contamination from cell lysis. Among the tested methods, CER resulted in EPS extraction with minimal contamination. Therefore, we propose that CER is currently the most appropriate method for extraction of EPS from Ultisols.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.