Extraction of diverse polyphenols in relation with storage periods of Citrus paradisi CV. Shamber through HPLC-DAD technique using different solvent.

Abstract

In this article, Citrus paradisi, (Shamber) an exceptional source of Vitamins A and C and full of nutrients, selected for extraction of diverse polyphenols including dietary flavonoids and essential flavonoids by HPLC-DAD technique using various solvents. These essential targeted compounds also analyze after keeping different storage periods and compare with fresh fruits for better efficacy of these compounds. The highest number of phenolic compounds including gallic acid, chlorogenic acid sinapic acid, ferulic acid, myricetin, quercetin, and kaempferol extracted in methanol solvent leading to the new compounds of tetra-O-methylscutellar and heptamethoxy flavone. The essential flavonoids determined by polyethersulfone filter and insoluble precipitation separated by the dimethyl sulfoxide. The results showed that the methanolic extraction exhibited higher essential flavonoids including nobiletin, sinensetin, tangeritin, and tetra-O-methylscutellarein and heptamethoxy flavone. The RP-HPLC analysis exposed the maximum number of nutritional flavonoids like naringin, hesperidin, total flavones, glycosyl. Moreover, it observed that dietary flavonoids and phenolic compounds of stored fruits were unaffected in 30days of storage periods while minor variations were pragmatic during 60-90days storage. The investigation revealed that C. paradisi proves to be the valuable resource of different phenolic compounds and flavonoids which are effective against various oxidative stresses in the human body.