Abstract
Abstract A distinct feature of certain plant species growing on serpentinite derived soils is their ability to hyperaccumulate heavy metals such as nickel (Ni) and chromium (Cr). This study was conducted to determine the availability of these heavy metals and their ease of extraction with various reagents. Various standard chemical reagents were used to extract Cr and Ni from nine different soils originating from two sites in the eastern Transvaal, South Africa. In addition, the sequential extraction of Cr, Ni, and iron (Fe) was undertaken. Of all the extradants used 4M nitric acid was the most efficient. Acetic acid appeared to be the best single extradant for estimating plant available Cr on the basis of leaf concentration. Acetic acid, ammonium acetate, copper (II) chloride, EDTA and aluminium chloride appeared to be equally efficient for estimating plant available Ni. Oxidation of organic matter resulted in a higher recovery of Cr and Ni thus highlighting the importance of this fraction in immobilizin...
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