Abstract
This work aimed to prepare breviscapine from Erigeron breviscapus and investigate its protection mechanism on uterine ischemia-reperfusion injury (UIRI) in rats. Breviscapine was extracted and separated from Erigeron breviscapus. Sixty female SD rats were randomly divided into sham-operated, model and breviscapine groups. The breviscapine group was treated with 5 mg/kg breviscapine for three days. Then, the UIRI model was established in model and breviscapine groups via uterine artery ischemia for 30 min followed by reperfusion for 60 min. At the end of reperfusion, compared with model group, in breviscapine group the uterine tissue superoxide dismutase activity was increased, and the malondialdehyde level was decreased; the uterine tissue tumor necrosis factor α, interleukin (IL)-1β and IL-6 levels were decreased, and the IL-10 level was increased; the uterine tissue adenosine triphosphate, adenosine diphosphate, adenosine monophosphate and total adenine nucleotides levels were increased; the uterine cell apoptosis rate was decreased, the Bcl-2 protein expression level was increased, and the Bax protein expression level was decreased. In conclusion, breviscapine from Erigeron breviscapus can reduce the oxidative stress, inflammatory reaction, energy metabolism disorder and apoptosis in uterine tissue, thus exerting the protective effect on UIRI in rats.
Highlights
Ischemia reperfusion can cause the further aggravation of injury, which leads to the necrosis and dysfunction of involved tissues and organs, and eventually results in the infection, sepsis, multiple organ failure and other complications (Meyer et al, 1998)
3.1 Comparison of superoxide dismutase (SOD) activity and MDA level in uterine tissue among three groups
Results of this study showed that, compared with model group, in breviscapine group the uterine tissue tumor necrosis factor α (TNF-α), IL-1β and IL-6 levels were decreased, and the IL-10 level was increased
Summary
Ischemia reperfusion can cause the further aggravation of injury, which leads to the necrosis and dysfunction of involved tissues and organs, and eventually results in the infection, sepsis, multiple organ failure and other complications (Meyer et al, 1998). Breviscapine has the protective effect on ischemia-reperfusion injury of heart (Wang et al, 2013), liver (Lin et al, 2016) and other organs. Whether breviscapine has a protective effect on UIRI has not been reported. The oxidative stress, inflammatory reaction, energy metabolism disorder and apoptosis are often involved in the ischemia-reperfusion injury (Kohli et al, 1999; Yassin et al, 2002; Wang et al, 2006; Zhao et al, 2016). We extracted and purified breviscapine from Erigeron breviscapus, and investigated its protection mechanism on oxidative stress, inflammation, energy metabolism disorder and apoptosis in rats with uterine ischemia-reperfusion injury
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