Extraction of baicalin from Scutellaria baicalensis Georgi and its alleviative effect on acute respiratory distress syndrome in rats

Hongju Bao,Xianrong Du,Daguo Zhao

doi:10.1590/fst.29421

Abstract

In this study baicalin was extracted from Scutellaria baicalensis Georgi and applied to alleviate the acute respiratory distress syndrome (ARDS) in rats. The baicalin with 89.53% purity was successfully extracted from Scutellaria baicalensis Georgi. Sixty-five rats were randomly divided into control, model and low-, middle- and high-dose baicalin groups. The oleic acid-induced ARDS model was established in model and baicalin groups. The low-, middle- and high-dose baicalin groups were treated with 100, 200 and 400 mg/kg baicalin, respectively. After 3 h from treatment, compared with model group, in baicalin groups the arterial oxygen partial pressure and oxygenation index were obviously enhanced, the left lung ratio wet weight to dry weight and number of neutrophil, total protein content, tumor necrosis factor α, interleukin 1β and interleukin 6 levels in bronchoalveolar lavage fluid were significantly decreased, and the lung tissue high-mobility group box-1 (HMGB1) and nuclear factor kappa B (NF-κB) p65 protein expression levels were significantly decreased. In conclusion, baicalin may alleviate the ARDS in rats by reducing the inflammatory response via inhibiting the HMGB1/NF-κB signal pathway.

Highlights

Many natural products have the anti-inflammatory activities (Amaral et al, 2017; Felhi et al, 2017; Lee et al, 2019; Costa et al, 2020)
Baicalin (C21H18O11) is one of the effective components of Scutellaria baicalensis Georgi, a herbal medicine commonly used in China
Previous studies have shown that the lung injury is related to the activation of high-mobility group box-1 (HMGB1)

Summary

Introduction

Many natural products have the anti-inflammatory activities (Amaral et al, 2017; Felhi et al, 2017; Lee et al, 2019; Costa et al, 2020). Baicalin can ameliorate the lipopolysaccharide‐induced acute lung injury in mice by suppressing oxidative stress and inflammation via the activation of the nuclear erythroid factor 2-mediated heme oxygenase-1 signaling pathway (Ranieri et al, 1999). Acute respiratory distress syndrome (ARDS) is a pulmonary inflammatory response caused by excessive release of inflammatory factors. How to inhibit the inflammatory response for alleviating ARDS has become a research hotspot (Reid & Donnelly, 1996). Previous studies have shown that the lung injury is related to the activation of high-mobility group box-1 (HMGB1). HMGB1 can further activate the nuclear factor kappa B (NF-κB) signal pathway, so as to release a large number of inflammatory mediators and cause the lung injury (Lan et al, 2017). The oleic acid‐induced ARDS model of rats was established, and the alleviative effect of baicalin on ARDS and its relation with

Methods

Results

Conclusion