Alleviation of Lipopolysaccharide-Induced Acute Respiratory Distress Syndrome in Rats by Yiqi Huayu Jiedu Decoction: A Tandem Mass Tag-Based Proteomics Study.

Chang-Yong Luo,Xin Li,Wei Sun,Yan Li,Cai-Hua Xiong,Qian Wang,Yuan-Hong Ma,Xu Liang,Xin Wang,Yan-Peng Zeng

doi:10.3389/fphar.2020.01215

Abstract

BackgroundTo study the effect of Yiqi Huayu Jiedu Decoction (YQHYJD) on protein expression in the lung tissue of acute respiratory distress syndrome (ARDS) rats and to explore the underlying molecular therapeutic mechanism of YQHYJD.MethodsSprague Dawley rats were administered with YQHYJD by oral gavage for 1 week. The rats were injected with lipopolysaccharide (LPS) to induce ARDS. The lung injury was assessed pathologically. Differentially expressed proteins (DEPs) were screened by quantitative proteomics and analyzed using bioinformatic tools, such as Metascape and Kyoto Encyclopedia of Genes and Genomes (KEGG) mapper. DEPs were verified by parallel reaction monitoring (PRM).ResultsYQHYJD alleviated the LPS-induced pathological damage of lung tissue in rats. There were 134 DEPs among the YQHYJD treatment and model groups. The Genomes pathway analyses revealed that the DEPs were closely related to immune system pathway. The mass spectrometry analysis revealed that YQHYJD exhibits a protective effect on lung tissue by significantly upregulating hematopoietic cell kinase (Hck), phospholipid phosphatase 3 (Plpp3), myristoylated-alanine rich C-kinase substrate (Marcks), and Actin-related protein 2/3 complex subunit 2 (Arpc2), which are related to Fc gamma receptor-mediated phagocytosis pathway.ConclusionYQHYJD can alleviate the lung injury of ARDS rats by regulating the Fc gamma receptor-mediated phagocytosis pathway, which is related to immune system.

Highlights

Acute respiratory distress syndrome (ARDS) is a clinical condition characterized by progressive hypoxemia and respiratory distress
The mass spectrometry analysis revealed that Yiqi Huayu Jiedu Decoction (YQHYJD) exhibits a protective effect on lung tissue by significantly upregulating hematopoietic cell kinase (Hck), phospholipid phosphatase 3 (Plpp3), myristoylated-alanine rich C-kinase substrate (Marcks), and Actin-related protein 2/3 complex subunit 2 (Arpc2), which are related to Fc gamma receptor-mediated phagocytosis pathway
YQHYJD can alleviate the lung injury of ARDS rats by regulating the Fc gamma receptor-mediated phagocytosis pathway, which is related to immune system

Summary

Introduction

Acute respiratory distress syndrome (ARDS) is a clinical condition characterized by progressive hypoxemia and respiratory distress. The histological characteristics of ARDS include acute diffuse inflammatory lung injury, which results in decreased barrier function of alveolar capillary endothelial cells, increased permeability, and exudation of a large amount of protein, red blood cells, and liquids. This leads to osmotic pulmonary edema and transparent membrane formation, which markedly affect ventilation (Thompson et al, 2017). To study the effect of Yiqi Huayu Jiedu Decoction (YQHYJD) on protein expression in the lung tissue of acute respiratory distress syndrome (ARDS) rats and to explore the underlying molecular therapeutic mechanism of YQHYJD

Methods

Results

Discussion

Conclusion