Extraction, Isolation, Phytochemical Screening and Evaluation of Anti Oxidant and Hypoglycemic Activity of Methanolic Extract of Tagetes Patula Linn Flowers

Abstract

Tagetes patula L. flowers possess different therapeutic properties, because of the presence of phytochemicals like terpenoids, flavonoids and thiophenes. The main objective of this study was to do extraction, followed by phytochemical screening, isolation and evaluation of antidiabetic and anti-oxidant activities of methanolic extract of Tagetes patula L. flowers. The evaluation of antidiabetic activity was carried out by α–amylase inhibition method and the anti-oxidant activity was evaluated hydrogen peroxide scavenging assay and nitric oxide scavenging method. The crude methanolic extract and selected column fractions of Tagetes patula L. flowers have shown anti-oxidant activity. In case of hydrogen peroxide scavenging assay the IC50 value was 8.9 µg mL-1 and in nitric oxide scavenging method it was 31.7 µg mL-1. The IC50 value for antidiabetic activity was 220 µg mL-1. Both the crude methanolic extract and selected column fractions of Tagetes patula L. flowers shown significant antidiabetic and anti-oxidant activity when compared with standards.