Abstract
Extraction of Omega-3 fatty acid from Catfish using enzymatic hydrolysis techniques are expected to be more economically possible techniques due to the uses of enzyme with the characteristic of reusable, environmental friendly and less energy required for large-scale production. Malaysian like to consume Catfish in their daily diet. Thus, it is very popular among farmers in Malaysia. Omega-3 polyunsaturated fatty acid have many benefit to human health. Based on research, EPA and DHA can reduce the risk of heart disease by regulate the blood coagulation. The main purpose of this research is to extract the Omega-3 polyunsaturated fatty acid by using enzymatic hydrolysis method. Enzymatic extraction technique by using Alcalase was used to extract the Catfish oil. Then, lipase was used to concentrate the omega-3 polyunsaturated fatty acid. Peroxide value and acid value were used to determine the Catfish oil quality. Optimization using DOE verify that the highest percentage of oil yields at 51°C, 0.91% enzyme concentration and 155 minute incubation time. Finally, there are two types of omega-3 polyunsaturated fatty acid can be found in Catfish oil which is EPA and ALA.
Highlights
Catfish is one of the diverse group of ray-finned fish that mostly found in the freshwater of Malaysia
This experiment operated in two main sections: - Optimized the optimum condition for extraction of the highest percentage of oil yields. - Concentrated the omega-3 fatty acid from fish oil
Temperature, enzyme concentration, and incubation time is the significant parameter which will affect the oil yields percentage obtained from the experiment
Summary
Catfish is one of the diverse group of ray-finned fish that mostly found in the freshwater of Malaysia. Catfish is grey in colour and scaless. People can identify catfish by their long whiskers-like barbels extend from their mouth. Catfish may consist up to four pairs of barbels. Catfish consists of flattened broad head and hollow bony leading ray on their dorsal and pectoral fins. They are usually bottom feeder and they like to prey at night. Catfish are carnivores so they mostly prey small fish, worms, frogs, insects, and newts that present in the river [1]
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