Abstract
Characteristics of collagen are influenced by the source of raw materials and extraction methods used. The aim of this research was to characterize the acid- and pepsin-soluble collagens from the dry scales of the striped snakehead (Channa striatus). Collagen was extracted using to methods including 0.5 M acetic acid and 0.1% pepsin. The yield of acid soluble collagen (KLA-SH) and pepsin soluble collagen (KLP-SH) were 0.98% and 1.94%, respectively. KLA-SH and KLP-SH contained glycine as the major amino acid and had high imino acid group content i.e 226 and 230 residues/1.000 residues, respectively. FTIR spectra of KLA-SH and KLP-SH showed that of the structure of collagen could be maintained in the form of triple helix structure. KLA-SH and KLP-SH consisted of α1- and α2-chain, β-chain, and γ-chain and is suggested as type I collagen.
Highlights
Kolagen merupakan bagian dari matrik ekstraselular protein yang berperan penting dalam mempertahankan struktur berbagai jaringan, berupa komponen utama dari berbagai jaringan ikat seperti kulit, tulang, tendon, pembuluh darah, membran basal, tulang rawan dan gigi yang menyumbang sekitar 25-30% dari total kandungan protein hewani (Liu et al 2007; Pati et al 2010)
Characteristics of collagen are influenced by the source of raw materials and extraction methods used
FTIR spectra of kolagen larut asam dari sisik haruan (KLA-SH) and kolagen larut pepsin dari sisik haruan (KLP-SH) showed that of the structure of collagen could be maintained in the form of triple helix structure
Summary
Bahan utama yang digunakan dalam penelitian ini berupa sisik haruan segar yang diperoleh dari pasar tradisional di Samarinda. Bahan-bahan yang digunakan untuk ekstraksi kolagen terdiri dari NaOH, EDTA, CH3COOH, NaCl (Merck), dan pepsin (EC3.4.23.1; powderized; ≥250 Units/mg solid) dari Sigma Chemical Co. (St. Louis, MO, USA), sedangkan bahanbahan untuk elektroforesis diperoleh dari Bio-Rad Laboratories (Hercules, CA, USA). Semua reagen dan bahan kimia lain adalah analytical grade. Alat-alat yang digunakan adalah refrigerated centrifuge (Eppendorf Centrifuge 5417 R, Hamburg, Germany), LC-MS/MS (The Waters Xevo TQD, Milford, MA), spektrofotometer UV-VIS (Genesys 10S, China), SDS-PAGE (Mini Protean unit, Bio-Rad Laboratories, Inc., Richmond, CA, USA), dan FTIR (Nicolet iS 10, Thermo Fisher Scientific Inc., USA)
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