Abstract
AbstractSeveral solvents were evaluated for extracting free long‐chain FA (LCFA) from a fermentation medium. Chloroform, chloroform/methanol (1∶1), hexane, and hexane/methyl tert‐butyl ether (MTBE) (1∶1) were evaluated as alternative extraction solvents. Parameters considered for optimizing LCFA recoveries included pH and ionic strength. Maximal LCFA recoveries were obtained by adding 2 mL of the hexane/MTBE (1∶1) solvent mixture, 80 μL of 50% H2SO4, and 0.05 g NaCl to 1 mL of the aqueous sample and mixing for 15 min at 200 rpm. This method quantified saturated LCFA [capric acid (C10∶0) to stearic acid (C18∶0)] and unsaturated LCFA with 18 carbons [linoleic acid (C18∶2) and oleic acid (C18∶1)] with a 98 to 100% recovery. Caproic (C6∶0) and caprylic (C8∶0) acids were characterized by 27 and 76% recoveries, respectively.
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