Extract Ethanol of Poguntano in Alloxan Induced Diabetic Rats

- Rusdiana Rusdiana,Sry Suryani Widjaja

doi:10.3329/bjms.v17i2.35879

- Rusdiana Rusdiana, Sry Suryani Widjaja

Open Access

https://doi.org/10.3329/bjms.v17i2.35879

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Abstract

Back Ground: Diabetes mellitus is a group of metabolic disease with high blood glucose level above 150 mg/dL over a prolonged period. The elevated of blood glucose, free fatty acid and insulin resistance will cause the endothelial dysfunction, hemostasis disturbances that lead to micro and macrovascular complications. Poguntano (Picria fel-terrae Merr) from family Scrophulariaceae found in most part of Indonesia, has been used as traditional plant for treatment of diabetes.Objective: The purpose of this study was to investigate the effect of extract ethanol of Poguntano in alloxan induced diabetic rats.Method: Fifteen male Wistar rats with body weight of 150-200 gr were given intra peritoneal injection of 150 mg/kg Alloxan to induce diabetes. These were divided into three groups (control diabetic, group given insulin and group given Extract ethanol of Poguntano 200 mg/Kg body weight) and one control normal control. The duration of study was 4 weeks; blood glucose and Endothelin-1 were measured for all groups.Results : Extract ethanol of Poguntano 200 mg showed significant results (p<0.001) in lowering blood glucose in Alloxan induced diabetic rats compared to control diabetic, but did not show superior to insulin group (p=0.892). Endothelin-1 showed statistical significant between group with normal rats and diabetic control rats. (p=0.009) but did not in the other groups. Levels of Endothelin-1 was higher in the diabetic control group with the median 1.95 (1,78- 3.1).Conclusion: In our study we found that extract ethanol of Poguntano showed significant hypoglycemic activity and lowering the Endothelin-1 level in alloxan induced diabetic rats.Bangladesh Journal of Medical Science Vol.17(2) 2018 p.251-254

Highlights

Diabetes Mellitus is a group of chronic metabolic disease resulting from the defect in insulin secretion or resistance with high blood glucose above 150 mg/dL.[1,2] The elevated of blood glucose, free fatty acid and insulin resistance will cause the endothelial dysfunction, hemostasis disturbances and later thrombosis[3]
Twenty wilstar male rats were divided into four groups: Group I normal control rats with no treatment Group 2 diabetic control rats with no treatment Group 3 diabetic rats received 1 unit of lantus Insulin Group 4 diabetic rats received oral extraxt ethanol Poguntano 200 mg/kg The duration of study was 4 weeks; blood glucose and Endothelin-1 were measured for all groups
This study showed the effect of lowering blood glucose in diabetic rats with extract ethanol

Summary

Introduction

Diabetes Mellitus is a group of chronic metabolic disease resulting from the defect in insulin secretion or resistance with high blood glucose above 150 mg/dL.[1,2] The elevated of blood glucose, free fatty acid and insulin resistance will cause the endothelial dysfunction, hemostasis disturbances and later thrombosis[3]. As the disease progress the activation of this endothelial system will leads to structural alteration, thrombosis and developed plaque in the vessel wall, fibrosis and inflammation[7,8,9] The activation of Endothelin-1 stimulate the proliferation of vascular smooth muscle cell.[9] These all suggested ET-1 might play a major role in diabetic vascular complications.[7,10] The mainstay treatments of diabetes are control diets, exercise and medicine 11,12,13 A variety of traditional medicine has been used empirically to treat diabetes and Indonesia is a country rich of plenty medicinal plants.[14,15] Poguntano (Picria fel-terrae Merr) from family Scrophulariaceae, one of the medicinal plants found in North Sumatera has been used to treat diabetes, fever, malaria and cancer, the anti hyperglycemic effect has been proved empirically.[16,17,18,19,20]

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