Abstract
Fish oil (FO) containing docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) has been reported to exert beneficial health effects on the host in the early course of Trypanosoma cruzi infection. T. cruzi virulence factors include molecules expressed on the cell surface as well as those secreted or shed into the extracellular medium. Extracellular vesicles shed by T. cruzi (EV) promote infection susceptibility of mammalian cells. We examined whether fish oil supplementation could prevent the effects of EV in the early course of T. cruzi infection. We show that the inoculation of EV from Y strain of T. cruzi (EV Y), in C57BL/6 mice prior to T. cruzi infection resulted in increased parasitemia, increased cardiac parasitism and decreased nitric oxide (NO) production. Supplementation of the animals with FO prevented the increase of parasitemia in animals pre‐treated with EV Y and impairs the action of EV Y on NO production by spleen cells stimulated with Tc‐Ag. These results suggest that EV Y modulate the host response in favor of the parasite and that the presence of n‐3 PUFAs interfere in the action exerted by EV Y. This is the first description of EV from T. cruzi with n‐3 PUFAS‐dependent effects.Support or Funding InformationThis work was supported by CNPq (Edital Universal 14‐2014), and Fundação Araucária (grant No. 419‐2009). MILM, NGZ, BFCL and HTS, received scholarships from Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). PPF received research fellowship from CNPq.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
Published Version
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