Abstract
Soil contamination by toxic heavy metals such as copper is a serious problem. In this study, the extracellular polymeric substance (EPS) extracted from Rahnella sp. LRP3 was found with the potential of immobilizing Cu-polluted in soil. The EPS could bond to Cu (II) through functional groups (polysaccharides, amide, proteins, and carboxyl groups), which further developed into the porous sphere with a diameter of 20 μm. Besides, EPS could induce the formation of Cu5(PO4)2(OH)4 crystal by the biomineralization process. Finally, the EPS in the culture solution reduced 89.4 mg/kg of DTPA-Cu content by 78.99% in soil for 10 d under the condition of 25 °C via biomineralization. The results demonstrated that EPS produced by Rahnella sp. LRP3 will be a promising factor in the remediation of Cu contaminated soil.
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