Abstract
Single-Pair Fluorescence Resonance Energy Transfer (FRET) experiments reveal structural and dynamic information about macro-molecules by monitoring the change in FRET efficiency between fluorescent dyes attached to a macro-molecule. The Nano-Positioning System (NPS) developed recently [1] uses data from several of such experiments to infer the position of a dye attached to protein sites unresolved by x-ray crystallography. Briefly, we perform probabilistic data analysis that allows us to calculate the distribution of possible dye positions in a simple and objective way without relying on ad-hoc procedures. Up to now NPS was limited to the triangulation of just one fluorescently labelled position based on FRET measurements to several other positions known from crystal structure [1,2]. Here, we discuss ways to extend the present model beyond this basic triangulation principle. In particular, we show how to gain three dimensional distance information by analysing triangulation networks where FRET is measured between arbitrary labelling sites in absence of other structural information. [1] A. Muschielok, J. Andrecka, A. Jawhari, F. Brückner, P. Cramer & J. Michaelis, Nat. Meth. 5, 965-971 (2008) [2] J. Andrecka, B. Treutlein, M.A. Izquierdo Arcusa, A. Muschielok, R. Lewis; A.C.M. Cheung, P. Cramer, and J. Michaelis, NAR doi:10.1093/nar/gkp601 (2009)
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