Abstract
ABSTRACT Gram-negative bacteria that produce Extended-spectrum beta-lactamase (ESBL) and metallo beta-lactamase (MBL) show resistance to beta-lactam antibiotics. This study was to determine Beta-lactamases-producing Pseudomonas species in fish ponds. Pseudomonas species isolated from aquaculture water samples using Pseudomonas base agar were characterised biochemically. The detection of ESBL and MBL was made by double disc synergy and imipenem-EDTA combined disc methods, respectively. Antimicrobial susceptibility was by disc diffusion method. The 94 Pseudomonas species isolated comprised P. aeruginosa (62.8%), P. stutzeri (14.9%), P. putida (9.8%) and P. fluorescens (12.8%). P. aeruginosa 16 (16.3%) and P. stutzeri 4 (4.3%) produced ESBL, but 2 (10%) P. aeruginosa and 1 (5%) P. stutzeri produced MBL. Resistance of ESBL producers to trimethoprim was 55.5% and 7 (35.0%) were multidrug resistant. Detection of ESBL and MBL in Pseudomonas spp. from this study implies environmental health risk. Antibiotics misuse in aquaculture and discharge of untreated aquaculture wastewater into the environment should be discouraged.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
