Abstract
ABSTRACT Extensive use of antibiotics leads to the appearance of multi-drug resistant (MDR) ESBL-producing bacteria across various sectors, however, little is known about their emergence from fish ponds. This study investigates the isolation of MDR ESBL-producing bacteria from three fish ponds in Ibadan, Nigeria, where water samples (n = 72) were collected and analysed for Gram-negative bacteria. Antibiotic susceptibility testing and ESBL phenotypic detection were performed using the Kirby-Bauer disc diffusion method and the double disc synergy test, respectively. Polymerase chain reaction identified ESBL bla SHV, bla TEM, and bla CTX-M genes. MDR ESBL-producers were confirmed through 16S rRNA sequencing. Pseudomonas species (n = 52), Plesiomonas species (n = 24), and Morganella species (n = 6) were isolated from the fish ponds, whereas Pseudomonas monteilii (n = 2), and one isolate each of Pseudomonas viridiflava and Plesiomonas shigelloides, were MDR. Morganella morganii (n = 1) was MDR-ESBL and harboured bla SHV and bla CTX-M. The presence of MDR ESBL-producing bacteria in fish pond water suggests aquaculture may threaten public health unless overuse of antibiotics can be prevented.
Published Version
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