Abstract
A new type of post-column flow cell with extended optical path length has been developed to improve sensitivity of UV-visible absorbance detection for capillary electrophoresis. In this flow cell, the outlet of the capillary column is connected vertically to the middle of a 3-mm microchannel. Auxiliary flows are employed to flush substances eluted from the outlets of the microchannel. The optical path length is the full length of the microchannel. An 8-fold signal-to-noise ratio improvement over on-column detection has been demonstrated. However, the post-column flow cell suffered 44% loss in resolution. A limit of detection of 3.2·10 −7 M has been obtained for fluorescein isothiocyanate.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
