Abstract
A new type of post-column flow cell with extended optical path length has been developed to improve sensitivity of UV-visible absorbance detection for capillary electrophoresis. In this flow cell, the outlet of the capillary column is connected vertically to the middle of a 3-mm microchannel. Auxiliary flows are employed to flush substances eluted from the outlets of the microchannel. The optical path length is the full length of the microchannel. An 8-fold signal-to-noise ratio improvement over on-column detection has been demonstrated. However, the post-column flow cell suffered 44% loss in resolution. A limit of detection of 3.2·10 −7 M has been obtained for fluorescein isothiocyanate.
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