Expressions of Topo IIα and Ki67 in breast cancer and its clinicopathologic features and prognosis.

Guangyu Sun Sun,Shuyan Wang,Ying Wang

doi:10.12669/pjms.35.3.81

Guangyu Sun Sun, Shuyan Wang + Show 1 more

Open Access

https://doi.org/10.12669/pjms.35.3.81

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Abstract

Objective:Breast cancer is one of the most common malignant tumors in women, and its incidence in women has ranked the first place among all malignant cancers. The evolution of breast cancer which is highly heterogeneous is a complex biological process involving multiple factors, genes and stages. Therefore, studying the expression and interaction of related genes is of great significance to further revealing of the mechanism of the occurrence and development of breast cancer and reduction of its resistance to chemotherapy. The purpose of this study was to investigate expressions of Ki67 and Topoisomerase IIα (Topo IIα) in breast cancer tissues and to explore the relationship between expressions of Ki67 and Topo IIα and the pathological characteristics and prognosis of breast cancer.Methods:Tissues with pathological changes and peripheral normal breast tissues were collected from 66 patients who underwent breast cancer treatment. Ki67 and Topo IIα in the breast cancer tissues and normal breast tissues were detected using immunohistochemical method. The relationships of Ki67 and Topo IIα with general clinicopathologic features and prognosis were analyzed.Results:It was found that the positive expressions of Ki67 and Topo IIα in the breast cancer tissues were higher than those in the normal tissues; the positive expression rate of Ki67 was in a correlation with diameter of breast cancer and lymphatic metastasis (P<0.05); the positive expression rate of Topo IIα was in a correlation with clinical stage, diameter of cancer and lymphatic metastasis (P<0.05). Multi-factor logistic regression analysis suggested that lymphatic metastasis and clinical stage were the independent predictive factors for positive expression of Topo IIα and lymphatic metastasis was the independent predictive factor for positive expression of Ki-67. Breast cancer patients with negative Ki67 and Topo IIα had significantly higher local recurrence rate than those who had positive Ki67 and Topo IIα (P<0.05) after surgery. The median time to progression (TTP) of the patients with positive expression of Topo IIα was lower than that with negative expression (P<0.05). The median TTP of the patients with negative and positive expression of Ki67 had no significant difference (P>0.05).Conclusion:Topo IIα and Ki67 can be used for reflecting the proliferation activity of cancer cells and can affect the postoperative recurrence and survival time after surgery. They may be involved in the occurrence, development and metastasis of breast cancer. They are expected to be indicators in the prognosis prediction of breast cancer and potential treatment targets.

Highlights

Breast cancer is one of the most common malignant tumors in women all over the world
There is no nationwide data on the incidence of breast cancer in China, but the predicted data show that there will be more than 100 cases of breast cancer among ten thousand women by 2021.4 A large number of studies have confirmed that mutations in key genes controlling cell growth play a leading role in the occurrence and development of breast cancer and the expression and level of some cell molecules can reflect the differentiation degree of tumor cells.[5,6]
The positive expression rate of Ki67 and Topo IIα in breast cancer tissues was 80.3% and 69.7% respectively (Fig.1), and both of them had no positive expression in normal breast tissues; the difference between the two groups was statistically significant (P

Summary

Introduction

Breast cancer is one of the most common malignant tumors in women all over the world. A survey shows that the incidence of breast cancer in developed countries in Europe and the United States is as high as 80/ten thousand.[3]. There is no nationwide data on the incidence of breast cancer in China, but the predicted data show that there will be more than 100 cases of breast cancer among ten thousand women by 2021.4 A large number of studies have confirmed that mutations in key genes controlling cell growth play a leading role in the occurrence and development of breast cancer and the expression and level of some cell molecules can reflect the differentiation degree of tumor cells.[5,6]. With the rapid development of molecular biology, more and more attentions have been paid to the study of tumor markers such as Ki-67 and Topoisomerase II α (Topo IIα) in breast cancer There is no nationwide data on the incidence of breast cancer in China, but the predicted data show that there will be more than 100 cases of breast cancer among ten thousand women by 2021.4 A large number of studies have confirmed that mutations in key genes controlling cell growth play a leading role in the occurrence and development of breast cancer and the expression and level of some cell molecules can reflect the differentiation degree of tumor cells.[5,6] With the rapid development of molecular biology, more and more attentions have been paid to the study of tumor markers such as Ki-67 and Topoisomerase II α (Topo IIα) in breast cancer

Methods

Results

Conclusion