Abstract
Objective:To investigate the expression of Topo IIα and Ki67 and its clinical significance.Methods:The clinical pathological data of one hundred and sixteen invasive breast cancer patients who were admitted into our hospital from July 2013 to December 2015 and underwent radical mastectomy were retrospectively analyzed. The expression of topoisomerase (Topo) IIα and Ki67 was detected using immunohistochemical method, and the correlation between the two kinds of proteins and the general clinical pathological characteristics of the patients was analyzed.Results:The positive expression rates of Topo IIα and Ki67 in breast cancer were 58.6% and 75% respectively. The expression of Topo IIα was in no apparent correlation with the age, tumor size, estrogen receptor (ER), progesterone receptor (PR) and human epidermal growth factor receptor 2 (HER-2) (P>0.05), but in a correlation with the number of metastatic lymph glands (P<0.05). The expression of Ki67 was in no apparent correlation with the age, tumor size, EP and HER-2, but in a correlation with the number of metastatic lymph glands and PR (P<0.05). The multi-factor logistic regression analysis results suggested that the number of metastatic lymph glands was the independent predictive factor of Topo IIα positive expression and the number of metastatic lymph glands and PR protein expression state are the independent predictive factors of Ki67 positive expression.Conclusion:Topo IIα and Ki67 can be regarded as the indicators for reflecting the proliferation activity of tumor cells, and the detection of Topo IIα and Ki67 expression is of great significance to the prognosis evaluation of breast cancer patients and clinical treatment.
Highlights
Breast cancer as a common malignant tumor has a high incidence among females, which can severely affect the health of human
The correlation between Topo IIα and Ki67 expression and clinical pathological characteristics: The positive expression of Topo IIα was in a correlation with the number of metastatic lymph glands, and the difference had statistical significance (X2=7.917, P=0.043); but it was in no correlation with age, tumor size, estrogen receptor (ER), progesterone receptor (PR) and HER2 (P>0.05; Table-I)
The results indicated that number of metastatic lymph glands was the independent predictive factor for the positive expression of Topo IIα, and number of metastatic lymph glands and PR protein expression were the independent predictive factors for the positive expression of Ki67 (Table-II)
Summary
Breast cancer as a common malignant tumor has a high incidence among females, which can severely affect the health of human. Recent studies have found that, multiple biological markers are in a close correlation with the prognosis of breast cancer.[4,5] Topoisomerase (Topo) IIα and Ki67 have been proved involving in the formation and development of tumor and can be regarded as the independent indicators for guiding clinical quality and indicating prognosis. Topo IIα is the target enzyme of anthracyclines, and its overexpression can reflect the proliferation state of cells.[6] Ki67 as the proliferating cell nuclear antigen (PCNA) for determining the proliferation state of tumor cells and prognosis has been extensively applied in clinics.[7]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
