Abstract
Objective To investigate the expressions of Toll-like receptor 4 (TLR4),nuclear factorkappa B (NF-κB) p65 and chemokine CCL20 in lesional and nonlesional skin of patients with progressive plaque-type psoriasis,as well as their clinical implications.Methods Paraffin-embedded tissue samples were prepared with lesional (n =40) and nonlesional (n =20) skin from progressive plaque-type psoriatic patients as well as from the normal skin of 10 human controls.Then,the expressions of TLR4,NF-κBp65 and CCL20 were detected by immunohistochemical staining technique using the EnVision antibody complex.Differences in the expression of these parameters were assessed by t test or corrected t test,and the correlations between these proteins were evaluated by Pearson's product-moment correlation coefficient.Results The expression levels of TLR4,NF-κBp65 and CCL20 were significantly higher in lesional skin than in nonlesional skin from patients and in normal skin from normal controls (TLR4:0.3006 ± 0.1200 vs.0.1930 ± 0.0687 and 0.1379 ± 0.0663,t =5.044,4.113,respectively,both P ＜ 0.05; NF-κBp65:0.4551 ± 0.1572 vs.0.2626 ± 0.0606 and 0.1970 ± 0.0679,t =3.711,6.106,respectively,both P ＜ 0.05; CCL20:0.3862 ± 0.1304 vs.0.2387 ± 0.0467 and 0.1262 ± 0.0612,t =4.889,5.265,respectively,both P ＜ 0.05),and higher in nonlesional skin of patients than in normal skin of the controls (t =2.118,2.685,5.608,respectively,all P ＜ 0.05).Moreover,the expressions of TLR4,NF-κBp65 and CCL20 were positively correlated with each other in lesional skin of the patients (r =0.766,0.851,0.885,respectively,all P ＜ 0.05).Conclusion The up-regulated expressions of TLR4,NF-κBp65 and CCL20 might be involved in the pathogenesis of psoriasis. Key words: Psoriasis; Toll-like receptor 4; NF-κBp65; CCL20
Published Version
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