Abstract
ObjectiveTo investigate the expression level and potential mechanism of hypoxia‐inducible factor 1 alpha (HIF‐1α) in patients with myelodysplastic syndromes (MDS).MethodsImmunohistochemistry (IHC) techniques were used to examine the protein expression of HIF‐1α in paraffin‐embedded myeloid tissues from 82 patients with MDS and 33 controls (patients with lymphoma that is not invading myeloid tissues). In addition, the associations between the protein expression of HIF‐1α and clinical parameters were examined. To further investigate the significance of HIF‐1α expression in MDS patients, the researchers not only extracted the data about HIF‐1α expression from MDS‐related microarrays but also analyzed the correlation between the level of HIF‐1α expression and MDS. The microRNA (miRNA) targeting HIF‐1α was predicted and verified with a dual luciferase experiment.ResultsImmunohistochemistry revealed that the positive expression rate of HIF‐1α in the bone marrow of patients with MDS was 90.24%. This rate was remarkably higher than that of the controls (72.73%) and was statistically significant (P < .05), which indicated that HIF‐1α was upregulated in the myeloid tissues of MDS patients. For the GSE2779, GSE18366, GSE41130, and GSE61853 microarrays, the average expression of HIF‐1α in MDS patients was higher than in the controls. Particularly for the GSE18366 microarray, HIF‐1α expression was considerably higher in MDS patients than in the controls (P < .05). It was predicted that miR‐93‐5p had a site for binding with HIF‐1α, and a dual luciferase experiment confirmed that miR‐93‐5p could bind with HIF‐1α.ConclusionThe upregulated expression of HIF‐1α was examined in the myeloid tissues of MDS patients. The presence of HIF‐1α (+) suggested an unsatisfactory prognosis for patients, which could assist in the diagnosis of MDS. In addition, miR‐93‐5p could bind to HIF‐1α by targeting, showing its potential to be the target of HIF‐1α in MDS.
Highlights
Myelodysplastic syndromes (MDS) are defined as a category of clonal myeloid disorders that are prone to lead the development of acute myelocytic leukemia (AML).[1,2,3,4] Currently, researchers are not very knowledgeable about the complex pathogenesis of MDS
IHC staining was conducted on myeloid tissues from 82 MDS patients and 33 controls, and hypoxia‐inducible factor 1 alpha (HIF‐1α) expression was evaluated in three types of tissues (Figures 1-3)
The researchers observed that the positive expression rate of HIF‐1α was higher in the RAEB1/RAEB2 cohort than in the RA/RARS/ RCMD/5q cohort, the rate of HIF‐1α (+) was higher in the initial cell ≥5% cohort than in the initial cell
Summary
Natural Science Foundation of Guangxi Province, Grant/Award Number: 2011GXNSFA018256 and 2015GXNSFDA139028; Guangxi Education Department Research Project, Grant/Award Number: ZD2014033; First Affiliated Hospital of Guangxi Medical University; Guangxi Feasible Technology Research and Development of Medical Treatment and Public Health
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
