Abstract
Objective To detect differentially expressed long non-coding RNAs (lncRNAs) and mRNAs in atrial tissues from patients with atrial fibrillation (AF) using microarray and to explore the role of lncRNAs in AF. Methods To use microarray technology to test the different of lncRNAs and mRNAs expression in 3 cases of atrial tissues and 3 cases of normal tissues with Agilent human lncRNAs Array and screened differentially expressed genes. Meanwhile, GO and Pathway analysis was performed. Using real-time quantitative reverse transcription polymerase chain reaction (qRT-PCR) to identified and an internal reference of GAPDH. By calculating co-express coding genes of lncRNAs and transcription factors, using hypergeometric distribution to calculate the degree of enrichment and found relevant transcription factor to construct the coding-non-coding gene co-expression network. Results A total of 219 lncRNAs and 197 mRNAs were found to be differentially expressed between the AF cases and normal atrial cases, 156 of the lncRNAs were up-regulated and 63 of the lncRNAs were down-regulated. Meanwhile, 120 of the mRNAs were up-regulated and 77 mRNAs were down-regulated. GO Term enrichment in the differentially expressed mRNAs was mainly in protein tyrosine kinase activity, calcium ion binding, DNA binding et al. Differentially expressed mRNAs may involve in Cell adhesion molecules, NF-Kappa B signaling pathway, Ras signaling pathway and so on. Conclusion The study found that a large number of differential expression of lncRNAs and mRNAs and which may have a close correlation with the pathogenesis of AF. Key words: Atrial fibrillation; Long non-coding RNA; Messager RNA
Published Version
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