Expression of Sonic Hedgehog in non-small cell lung cancer and its relationship with the cycle of life

Abstract

Objective To observe the expression of Sonic Hedgehog (Shh) signaling pathway related protein in patients with non-small cell lung cancer and explore their roles in the development of lung cancer.Methods Forty-six patients with non-small cell lung cancer undergoing surgical operation were selected in our hospital from January to June,2013,which were operation resection of lung cancer as the observation group,39 cases the tissue of adjacent to carcinoma,28 cases of lung tissue with no lung cancer as control group.Using immunohistochemical method to observe the Shh and Gli2 protein expression.Results Shh and Gli1 protein in the cells were expressed in the cytoplasm under light microscope,yellow or brown yellow.The positive rate of Shh and Gli1 protein in the observe group were respectively 73.91％ (34/46) and 67.40％ (31/46),were higher than that in paracancerous tissues and the control group (P ＜0.05),The positive rate of Shh and Gli1 protein in Ⅲ,ⅣWstage were 80.95％ (17/21),71.43％(15/21) and 91.67％ (11/12),83.33％ (10/12),were higher than those of Ⅰ and Ⅱ phase (P ＜ 0.05).Conclusion In Shh signal pathway,there may be through the activation of Shh protein and the expression of Gli protein which promote carcinogenesis and cell proliferation in non-small cell lung cancer. Key words: Sonic Hedgehog pathway; Non small cell lung cancer; Shh protein; Gli1 protein