Expression of p120-catenin in different bladder cancer cells and effect of p120-catenin to the prolifer-ation, invasion and adhesion of human bladder carcinoma cell line BIU-87 in vitro

Abstract

Objective To investigate the expression of p 120-catenin in human bladder cell line BIU-87 and T24.And to explore the effect of down-regulation of p120-catenin on proliferation, invasion and adhesion of BIU-87 cell line. Methods The study was from Aug .2010 to May.2011.Real-time RT-PCR and Western blot were used to examine the expression of p 120-catenin in human bladder cell line BIU-87 and T24.There were three study groups：non-transfected group , the group transfected with p 120-catenin siRNA and the group transfected with control siRNA .p120-catenin siRNA was used to decrease the expression of p120-catenin.MTT assay and colony formation assay were used to study the BIU-87 cell growth and cell pro-liferation.The invasion of BIU-87 cells was measured by Transwell chamber assay .Cell adhesion artificial re-constituted basement membrane assay was used to examine the change of BIU-87 cell adhesion capacity . Results In both BIU-87 and T24 cells there were the expressions of p 120-catenin.But the expression in BIU-87 cells （0.11±0.39） was more than that in T24 cells （0.48±0.17）.The decreased of p120-catenin ex-pression could enhance the proliferation （182.7±13.4%） and the invasiveness （217.5±15.9） and decrease the adhesion capacity （57.3±6.4%） of BIU-87 cells. Conclusions There is higher expression level of p120-catenin in lower-grade malignant bladder cancer cells .The down-regulation of p120-catenin promotes the bladder cancer proliferation and invasiveness of bladder carcinoma cells , and inhibited the bladder canc-er cell adhesion . Key words: p120-catenin; BIU-87 cell; Proliferation; Invasion; Adhesion