Expression of phosphatases of regenerating liver-1 and phosphatases of regenerating liver-3 in the bladder transitional cell carcinoma cells

Abstract

Objective To study the expression of phosphatases of regenerating liver (PRL)-1 and PRL-3 in the different bladder transitional cell carcinoma lines, so as to detect their roles in invasion and metastasis of cancer. Methods Reverse transcription polymerase chain reaction (RT-PCR) and immunocytochemistry were used to detect the expression levels of PRL-1 and PRL-3 in the BIU-87 and T24 cell lines. Boyden chamber experiment was used to detect the invasiveness of the two cell lines. Results The relative transcript levels of PRL-1 and PRL-3 mRNA were 0.772±0.037 and 0.304±0.033 in the BIU-87 cells, and 0.828±0.039 and 0.298±0.038 in the T24 cells (t=20.930 and 21.494, P=0.000). The relative transcript levels of PRL-1 and PRL-3 mRNA had the positive correlations in the BIU-87 cells and the T24 cells (r=0.941, P=0.017; r=0.997, P=0.000). The relative transcript levels of PRL-1 and PRL-3 protein were 7.000±1.870 and 4.400±1.140 in the BIU-87 cells, and 8.200±0.836 and 5.200±1.303 in the T24 cells (t=2.654, P=0.029; t=4.330, P=0.003). The relative transcript levels of PRL-1 and PRL-3 protein had the positive correlations in the BIU-87 cells and the T24 cells (r=0.958, P=0.010; r=0.942, P=0.017). The transmembrane cell counts of the BIU-87 and T24 cells were respectively (148.800±6.418) and (102.200±6.340) cells with the difference being significant (t=11.549, P=0.000). Conclusion High expression levels of PRL-1 and PRL-3 are observed in the bladder transitional cell carcinoma cell lines, and they maybe take a very important part in the invasion and metastasis of bladder transitional cell carcinoma. Key words: Phosphatases of regenerating liver-1; Phosphatases of regenerating liver-3; Bladder transitional cell carcinoma; Invasion; Metastasis