Expression of ovate family protein 8 affects epicuticular waxes accumulation in Arabidopsis thaliana

Yao Tang,Ying Chang,Peng Feng,Hong-Ling Li,Wei Zhang,Yan-Li Yin

doi:10.1186/s40529-018-0228-8

Abstract

BackgroundTranscription factors could regulate multiple aspects of plants growth and development, which is significant to plants. Ovate family proteins (OFPs) that are named due to contain OVATE domain, a 70-AA C-terminal conserved domain from the protein OVATE gene encodes, are plant-specific transcription factors family. Some members of OFPs have been shown to function as transcription factors to regulate plant growth and development, but little is known about the function of AtOFP8.ResultsHere, we found AtOFP8 maybe involve in transcriptional regulation of the epicuticular waxes in Arabidopsis thaliana. First, we observed that the distribution of epicuticular waxes of wild type plants was more than that of Atofp8-1 deletion mutants, but less than that of 35S:HA-AtOFP8 transgenic overexpression lines not only on the leaves but also on the stems utilizing scanning electron microscopes. Second, we extracted waxes from leaves and stems of three types of plants respectively to measure the waxes content and composition by gas chromatography–mass spectrometer (GC–MS), and the results of the total content of waxes were consistent with the results of scanning electron microscopes. Finally, we found that the expression of 12 genes related to the synthesis and metabolism of waxes was changed in the Atofp8-1 mutants and 35S:HA-AtOFP8 transgenic lines compared with wild type plants.ConclusionsThese findings suggest that AtOFP8 could change the accumulation of epicuticular waxes.

Highlights

Transcription factors could regulate multiple aspects of plants growth and development, which is significant to plants
We reported the difference of the shape and the content of epicuticular waxes by comparing Atofp81 mutants, wild-type plants and 35S:HA-AtOFP8 compared with Col-0. FC (35S):HA-AtOFP8 transgenic lines
Gene chip results showed that the transcription level of AtOFP8 in 35S:HA-AtOFP8 overexpression plants increased more than 300-fold, while decreased by nearly 14-fold in the Atofp8-1 mutant (Table 2)

Summary

Introduction

Transcription factors could regulate multiple aspects of plants growth and development, which is significant to plants. Ovate family proteins (OFPs) that are named due to contain OVATE domain, a 70-AA C-terminal conserved domain from the protein OVATE gene encodes, are plant-specific transcription factors family. A. thaliana ovate family proteins (AtOFPs) have been proved to be a novel transcriptional factor family, which could control multiple aspects of plant growth. Epicuticular waxes are complex mixtures of very long chain fatty acids (VLCFAs) and their derivatives, which are significant to plants (Shepherd and Griffiths 2006). They could form an important protective layer to protect the internal structure of the plant body (Koch and Ensikat 2008). Researchers generally believe that if stems or leaves have a glossy green phenotype, it is indicative of waxes deficiency, and if plants have whitish green stems, it is most likely displayed more waxes (Go et al 2014)

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Conclusion