Abstract
Neural cell adhesion molecule (NCAM) is important for cell migration and it could be expressed in some renal cell carcinoma (RCC). In recent decades, the incidence of RCC has been steadily rising by 2-4% each year. In this study NCAM expression and correlation with nuclear grade in different RCC were analyzed. We analyzed NCAM expression on 7 different RCC cell lines and 32 different RCC by immunohistochemistry, immunofluorescence, Western blot and FACS analysis. NCAM expression is detected in 6 cell lines and 16 RCC cases. NCAM-140 kDa isoform is expressed in different RCC and RCC cell lines. NCAM expression in non-invasive clear cell RCC is lower than in clear cell RCC with high nuclear grade. Expression of NCAM is not exclusive for specific RCC type, so NCAM can not be used as a specific diagnostic marker for RCC. NCAM expression is in correlation with nuclear grade in clear cell RCC, suggesting that NCAM expression is involved in aggressive behavior and metastatic potential in RCC.
Highlights
Cell adhesion molecules, including immunoglobulines, play important role in tumor genesis and metastatic cascade[1,2]
Neural cell adhesion molecule (NCAM) expression is in correlation with nuclear grade in clear cell renal cell carcinoma (RCC), suggesting that NCAM expression is involved in aggressive behavior and metastatic potential in RCC
But strong staining on the cell surface of all tumor cells was detected in only 4 RCC (Figure 1a, b) whereas focal, moderate to strong expression of NCAM on large clusters of cells was present in 12 RCC
Summary
Cell adhesion molecules, including immunoglobulines, play important role in tumor genesis and metastatic cascade[1,2]. Neural cell adhesion molecule (NCAM), a member of the large immunoglobulin superfamily (IgSF) of cell surface glycoproteins, is encoded by a single copy gene on chromosome[11]. From clinical aspects NCAM is an important indicator of neuroendocrine differentiation of cells[5]. Uninduced as well as induced foetal renal mesenchymal cells are well known to widely express NCAM as specific cell surface antigen up to the perinatal period during kidney development in humans and rats. Induced mesenchymal cells rapidly loose expression of NCAM after mesenchymal-epithelial transformation on immature tubular cells during further differentiation of the nephron[6]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
