Abstract
Objective This Work is aimed at exploring the effect of microRNA (MiR)-608 on the function of nonsmall cell lung cancer (NSCLC) A549 cells and related mechanisms. Methods Blood samples of 106 NSCLC patients (experimental group) as well as 124 normal people (control group) were selected for relevant investigation. Polymerase chain reaction (PCR) as well as DNA sequencing was used to determine the genotyping of the MiR-608 rs4919510 polymorphism. MiR-608 expression in cells was detected by real-time PCR technology. Western blotting was used to detect changes in protein levels. NSCLC tissues as well as adjacent tissues were explored in 33 patients undergoing surgery. Results MiR-608 rs4919510 does not influence the incidence of NSCLC patients. In addition, MiR-608 expression was downregulated in the tumor tissue of NSCLC patients, while the transcription factor activating enhancer-binding protein 4 (TFAP4) expression was upregulated. MiR-608 promotes DOX- (Doxorubicin-) induced apoptosis by negatively regulating TFAP4 expression in NSCLC tissue. TFAP4 can significantly inhibit the migration of A549 cells. Conclusion The findings in this investigation can contribute to the effective treatment of NSCLC patients. Also, the investigation can provide some theoretical support for the application of new targets for NSCLC treatment.
Highlights
Lung cancer is a kind of malignant tumor
Lung adenocarcinoma takes the largest proportion of all nonsmall cell lung cancer (NSCLC) [5]
Determining the correlation between the MiR-608 rs4919510 polymorphism site and NSCLC is the first step in this investigation
Summary
Lung cancer is a kind of malignant tumor. Its morbidity and mortality have the fastest growth. 50 years ago, in many countries, it was reported that the number of lung cancer patients increased rapidly. Among male patients, it is in the first place in all malignant tumors. 85% to 90% lung cancers are NSCLC, which become the most common cause of cancer death [4]. There is no clear conclusion about the cause and mechanism of lung cancer. In addition to environmental carcinogenic factors, there are other unknown reasons for the extremely high morbidity of lung cancer. MicroRNA (miRNA) is a large kind of endogenous noncoding single-stranded small molecule RNAs discovered in recent years. Hao et al (2017) reviewed the role of ectopic miRNAs in gastric cancer growth, migration, invasion, and apoptosis
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
