Abstract
Objective To investigate the expression of miR-27a-3p in tumor tissue of patients with non-small cell lung cancer (NSCLC) and explore the effect of miRNA-27a-3p expression on invasion and migration of lung cancer cells. Methods The tumor tissues of 21 NSCLC patients and corresponding adjacent normal tissues were collected for this study and the differences of miR-27a-3p expression levels in tumor tissues and adjacent tissues were detected by real-time fluorescence quantitative PCR. The expression of miR-27a-3p in A549 cells was overexpressed and silenced by transfecting miR-27a-3p mimics and miR-27a-3p inhibitor. And the ability of proliferation and migration of A549 cells after transfecting were detected by MTT and cell scratch assaies. Results The expression levels of miR-27a-3p in tumor tissues of NSCLC patients were significantly higher than adjacent non-cancerous tissues. The proliferation and migration ability of A549 cells were enhanced significantly after overexpression of miR-27a-3p. The silencing of miR-27a-3p expression in A549 cells produces the opposite effect. Conclusion Compared with normal tissues, NSCLC tissues have high expression of miR-27a-3p. Overexpression of miR-27a-3p enhanced the proliferation and migration ability of lung cancer cells. Therefore, miR-27a-3p may be an important regulatory factor in the development of NSCLC.
Highlights
Lung cancer was the malignant tumors with the highest morbidity and mortality around the world (Reck et al, 2017)
We found that miR-27a-3p expression were significantly increased in cells transfected with miR-27a-3p mimics compared with control group (Fig. 2A), whereas the expression levels of miR-27a-3p in cells transfected with miR-27a-3p inhibitor were obviously decreased compared with control group (Fig. 2B)
2.4 Effect of miR-27a-3p on Migration of A549 Cells We investigated the role of miR-27a-3p in the regulation of invasion of A549 cells
Summary
The tumor tissues of 21 NSCLC patients and corresponding adjacent normal tissues were collected for this study and the differences of miR-27a-3p expression levels in tumor tissues and adjacent tissues were detected by real-time fluorescence quantitative PCR. The expression of miR-27a-3p in A549 cells was overexpressed and silenced by transfecting miR-27a-3p mimics and miR-27a-3p inhibitor. The ability of proliferation and migration of A549 cells after transfecting were detected by MTT and cell scratch assaies
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