Abstract
Objective The aim of this research was to analyze the expression profile of miR-155, miR-146a, and miR-326 in peripheral blood mononuclear cells (PBMC) of 47 patients with type 1 diabetes mellitus (T1D) and 39 control subjects, as well as the possible association with autoimmune or inflammatory markers. Subjects and methods Expression profile of miRs by means of qPCR using TaqMan probes. Autoantibodies and inflammatory markers by ELISA. Statistical analysis using bivariate correlation. Results The analysis of the results shows an increase in the expression of miR-155 in T1D patients in basal conditions compared to the controls (p < 0.001) and a decreased expression level of miR-326 (p < 0.01) and miR-146a (p < 0.05) compared T1D patients to the controls. miR-155 was the only miRs associated with autoinmmunity (ZnT8) and inflammatory status (vCAM). Conclusion Our data show a possible role of miR-155 related to autoimmunity and inflammation in Chilean patients with T1D.
Highlights
Type 1 diabetes (T1D) is an autoimmune disease triggered by T cells that destroy pancreatic beta cells
The expression of miRNAs may be induced by a variety of stimuli-including cell stress and inflammation, which either induce or suppress its expression in response to different stimuli, which may influence some biological processes and have pro- or anti-inflammatory effects [5] – such as hyperglycemia in patients with T1D – which increases the inflammatory response by increasing cytokines. This effect is associated with increased expression of Toll receptors [6,7], and has been correlated with studies on peripheral blood mononuclear cells (PBMC) cultures stimulated with high glucose concentrations, which showed an increase in the expression levels of TNF-α, IL-1b, and IL-6 [8]
The relationship between miRNAs and the various components of the immune system has been addressed in different autoimmune pathologies previously. miR155 is related with the immune response of macrophages to different types of inflammatory mediators, such as TNF-α, which can induce the expression of miR-155 in macrophages and monocytes [14,15]. miR-146a is associated with innate immunity and inflammation [16]
Summary
Type 1 diabetes (T1D) is an autoimmune disease triggered by T cells that destroy pancreatic beta cells. This effect is associated with increased expression of Toll receptors [6,7], and has been correlated with studies on PBMC cultures stimulated with high glucose concentrations, which showed an increase in the expression levels of TNF-α, IL-1b, and IL-6 [8]. The aim of this study was to analyze the expression levels of the miRNAs miR146a, miR-155, and miR-326 in PBMC from T1D and healthy patients, and to estimate their possible relationships with inflammatory or autoimmunity status in Chilean children with T1D.
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