Expression of microRNA-chr1011 in hepatocellular carcinoma and its clinical significance

Abstract

Objective To observe the expression of microRNA (miRNA, miR)-chr1011 in hepatocellular carcinoma (HCC) tissues and analyze its clinical significance in HCC patients. Methods High-throughput deep sequencing was used to analyze the small RNA sequences in 6 pairs of HCC tissues. Data of deep sequencing were compared with the gene database to predict novel miRNAs by miRdeep 2.0.0.5 software. Northern blot was utilized to verify the structure and authenticity of the novel miRNAs. The expression levels of novel miRNAs in 62 pairs of HCC tissues were detected by Real-time quantitative polymerase chain reaction (RT-qPCR) and the clinical data of HCC patients were analyzed. Results MiR-chr1011 was found in human liver tissues and liver cancer cell lines, which has a stable precursor structure and is located on human chromosome chr10.11: 107803675-107803737: + . Northern blot assay confirmed its expression in HCCLM3 and Huh7 liver cancer cells. In 62 pairs of HCC tissues, the expression of miR-chr1011 in tumor adjacent tissues was higher than tumor tissues (4.37±0.31 vs. 2.81±0.21, P< 0.01), and the expression of miR-chr1011 was negatively correlated with the Barcelona stage of tumor (P<0.01), TNM stage (P<0.01) and portal vein thrombosis (PVTT) formation (P<0.05). Conclusion MiR-chr1011 is lowly expressed in liver cancer tissues and is significantly associated with the prognosis of HCC patients. Key words: Carcinoma, hepatocellular; MicroRNA-chr1011