Expression of M3 subtype of muscarinic receptors in normal,BPH and prostate cncer tissue

Abstract

Objective To study the relationship between M3 receptor and prostastic tumor by analyzing the expressions of M3 and vascular endothelial growth factor(VEGF)in adult human normal and neoplastic prostatic gland tissue. Methods The specimens included 36 normal prostates(fresh),36 benign prostatic hyperplasia(BPH)tissue(fresh),and 36 cancer tissue(8 fresh).RT-PCR was used to detect M3 receptor,VEGPs genetic expression.At protein level,VEGF,Ms receptor,CD34 were detected by western-blot and immunohistochemical method. Results VEGF and M3 receptor's genetic expressions were higher in prostate cancer tissue(O.8354±0.1897,0.7824±0.2047)than in BPH tissue(0.6735±0.1603,0.6021±0.1637),while the expressions of these genes were lowest in normal prostate tissue(0.5425±0.1629,0.3436±0.1581)(P<0.001).There was a positive correlation between M3 and VEGFs gene expression(r=0.4999,P<O.001).The protein expression of Ms receptor and VEGF was similar to their genetic expression pattern,the expressions in prostate cancer tissue(0.4777±0.1638,0.5718±0.2245)were higher than those in BPH tissue(0.3655±0.1474,0.4342±0.1538)and in normal prostate tissue(0.2659±0.1076,0.3380±0.1527)(P<0.05).Conclusions M3 receptor might be related with development of prostatic tumor.It could be used as a new target 0f diagnosis and treatment for prostastic tumor. Key words: Prostatic neoplasms; Prostatic hyperplasia; Receptor,muscarinic M3; MVD