Expression of integrin β 1 and its roles on adhesion between different cell cycle hepatocellular carcinoma cells (SMMC-7721) and human umbilical vein endothelial cells

Abstract

To investigate expression of integrin β 1 and its roles on adhesion between different cell cycle hepatocellular carcinoma cell (HCC) and human umbilical vein endothelial cells (HUVEC), the synchronous G 1 and S phase HCC were achieved through thymine-2-deoxyriboside and colchicines sequential blockage method and double thymine-2-deoxyriboside blockage method, respectively. Expression of integrin β 1 on hepatocellular carcinoma cells was detected with flow cytometer. Further, the adhesive force of HCC to HUVEC and the role of integrin β 1 in this adhesive course were studied by micropipette aspiration technique. The results showed that percentage of each cyclic phases of the controlled HCC (non-synchronous) are: G 2+M phase, 11%; G 1 phase, 54%; S phase, 36%; the synchronous rates of G 1 and S phase HCC amount to 74 and 98%, respectively. The expressive fluorescent intensity of integrin β 1 in G 1 phase HCC is depressed significantly than the values of S phase and controlled HCC. Accordingly, the adhesive forces of G 1 phase HCC to HUVEC was significantly lower than the value of S phase cells ( P<0.01), but it has no remarkable difference when compared the adhesive force values of S phase HCC with control; the contribution of integrin β 1 was about 50% in the adhesion of HCC to HUVEC. It suggested that HCC would be synchronized preferably in G 1 and S phase with thymine-2-deoxyriboside and colchicines, the adhesive molecule integrin β 1 expressed in a high lever in HCC and presented differences in vary cell cycle, and integrin β 1 played an important roles in adhesion of HCC to HUVEC. Possibly, S phase HCC take a great action in this adhesive course.