Expression of Human Papilloma Virus Infection, Proliferating Cell Nuclear Antigen and P53 in Cervical Squamous Cell Carcinoma

Abstract

Objective To explore the expression of human papilloma virus (HPV)infection, proliferating cell nuclear antigen (PCNA) and P53 immunoreactions in cervical squamous cell carcinoma (SCC) and their possible correlation with the carcinoma occurrence and development. Methods From October 2005 to November 2009, clinical data of 35 cervical squamous cell carcinoma cases(SCC group) and 13 cervical erosion cases (cervical erosion group) were reviewed and compared with their immune-pathological data of human papilloma virus infection and/or proliferating cell nuclear antigen and/or P53 expressions. This study was detected by immunohistochemistry SP methods in the carcinoma tissues. The study protocol was approved by the Ethical Review Board of Investigation in Human Being of Centre for Nuclear Industry 416 Hospital. Informed consent was obtained from all participants. Results The positive immunoreactive rates of human papilloma virus infection, proliferating cell nuclear antigen and P53 in SCC group were significantly higher than those of cervical erosion group (P<0.05). The strongest proliferating cell nuclear antigen expression was associated with the lower differentiation of pathological grades (P<0.05), but human papilloma virus infection and P53 were not related to the tumor grades. Conclusion Human papilloma virus infection and P53 may play important roles in the pathogenesis of cervical squamous cell carcinoma. The over expression of proliferating cell nuclear antigen may indicate the worse carcinoma pathological grades, that may help to predict the prognosis for cervical squamous cell carcinoma patients. Key words: human papilloma virus (HPV) infection; proliferating cell nuclear antigen (PCNA); P53; cervical squamous cell carcinoma