Abstract
Expression of gag-CA Gene of Jembrana Disease Virus with Cationic Liposomal and Chitosan Nanoparticle Delivery Systems as DNA Vaccine Candidates
Highlights
A prevention method has been introduced; A DNA vaccine candidate was developed by inserting the gag gene subunit Capsid (gag-CA) Jembrana Disease Virus (JDV) gene in the pEGFP-C1 vector and a delivery system using cationic liposomes and chitosan nanoparticles, which is an important factor in the success of DNA vaccines in inducing immunity
The results showed the design of recombinant DNA plasmids with cationic liposomal and chitosan nanoparticle delivery systems expressing the gag-CA gene JDV
The gene design was synthesised by Gene Universal Corp. inserted on the restriction side BglII and EcoRI on the pEGFP-C1 vector, forming a recombinant DNA plasmid with a base length of 5407 bp according to the Construct Map in Supplementary data 3
Summary
The fulfillment of people’s need for meat protein resources have to be balanced with the increase of livestock population. Bali cattle (Bos javanicus), as a local meat source, is expected to be the main beef source instead of imported livestock. The disease is caused by virus of family Retriviridae genus Lentiviridae (Kertayadnya et al 1993; Chadwick et al 1995), known as Jembrana Disease Virus (JDV) that has caused high mortality of Bali cattle across Indonesia for many decades. The limited supply of materials due to complicated, timeconsuming isolation techniques encourages experts to develop vaccines through recombinant DNA techniques. One of the recombinant DNA technology products is the DNA vaccine. This vaccine is made by inserting a DNA or gene that encodes an immunogenic protein into a eukaryotic expression vector
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