Expression of Foxq1 and NF-κB p65 in cervical cancer and its clinical pathological significance

Abstract

Objective:To screen for the transcription factor(TF) closely related to the development of cervical cancer tissue by microarrry gene chip and to testify the results at protein level,so as to assess clinico-pathologic significance of these TFs.Methods: The differential genes in 2 fresh cervical cancer specimens and their adjacent normal tissues and 2 normal cervical tissues were examined by Illumine Human-6 gene chip.The expression of the important TFs selected was verified by RT-PCR,and the major TFs regulating the differential genes were selected.The selected TFs were further investigated in 44 cervical cancer tissues,22 cervical intraepithelial neoplasia(CIN),and 17 normal cervical tissues using immunohistochemical method.The relationship between the expression of major TFs in cervical cancer and the clinico-pathological characteristics was evaluated.Results: There were 67 upregulated genes and 28 downregulated genes in cervical cancer tissue and CIN when compared to normal tissue,these were testified by RT-PCR.Among these genes,the expression of NF-κB p65 in cervical cancer tissues was up-regulated compared with that in the CIN tissues and normal tissues(P0.05).Expression of NF-κB p65 was positively correlated with the pathological grade of cervical cancer(P0.05),and was not associated with FIGO stage(P0.05).The expression of Foxq1 in cervical cancer was significantly lower than that in CIN and normal tissues(P0.05),and the expression was not correlated with FIGO stage,pathological grade,or NF-κB p65 expression(P0.05).Conclusion: NF-κB p65 and Foxq1 are associated with the development of cervical cancer;they may play different roles in the oncogenesis of cervical cancer.