Expression of Enhancer-Binding Protein CEBPA mRNA and Protein in Ovarian Cancer and Its Relationship With Pathobiological Characteristics

Chenchen Tian,Zhiting Dong,Minwen Fu,Limei Zhang,Shufang Mi,Mo Li

doi:10.3389/fsurg.2022.842823

Abstract

Ovarian cancer is a common malignant tumor, its early onset is hidden, lack of specific symptoms, the location of the lesion is particularly hidden, which makes it difficult to find ovarian lesions by general detection, making it difficult to make an early clinical diagnosis. Therefore, it is still the focus and difficulty of ovarian cancer research to find the means of early diagnosis and prognosis of ovarian cancer. Cytosine-cytosine-adenosine-adenosine-thymidine (CCAAT) enhancer-binding protein α (CEBPA) has been proved to be involved in cell metabolism, proliferation, and differentiation. In this study, the expression of CEBPA mRNA and protein in normal ovary, epithelial ovarian cyst, ovarian borderline tumor, and ovarian cancer was detected, the relationship between CEBPA and pathobiological characteristics of ovarian cancer was discussed, and its influence on the prognosis of patients with ovarian cancer was analyzed. The results showed that the expression of CEBPA mRNA and protein in patients with ovarian borderline tumor and ovarian cancer is high, and the expression of CEBPA has no obvious correlation with the pathobiological characteristics of patients with ovarian cancer, and the high expression of CEBPA has an important value in the diagnosis of ovarian cancer, and it is also a poor prognostic factor of the disease.

Highlights

Ovarian cancer is closely related to factors, such as heredity and hormone level
The results of real-time quantitative PCR showed that the expressions of C/EBP α [CCAAT enhancerbinding protein α (CEBPA) mRNA in ovarian tissues of patients with normal ovary, epithelial ovarian cyst, ovarian borderline tumor, and ovarian cancer were 0.95 ± 0.90, 1.08 ± 0.92, 3.56 ± 0.86, and 4.13 ± 0.87, respectively
Through real-time fluorescence quantitative PCR, western blotting (WB), immunohistochemistry, and other experiments, our results showed that compared with normal ovarian and epithelial ovarian cyst patients, the expression of CEBPA mRNA and protein in patients with ovarian borderline tumor and ovarian cancer were higher, which proves that the upregulation of CEBPA may play a role in the pathogenesis of ovarian cancer

Summary

Introduction

Ovarian cancer is closely related to factors, such as heredity and hormone level. It is a complicated process that involves many factors, genes, and steps, and the peak period of ovarian cancer is over 45 years old [1]. The clinical manifestations of ovarian cancer are mostly pain, and some patients may be accompanied by lower abdominal mass, irregular menstruation, etc. According to the global cancer report, in 2020, 4.43 million cases of cancer deaths among women worldwide, i.e., 210,000 cases of ovarian cancer [2]. The main features of ovarian cancer are high morbidity and mortality, early-onset concealment, lack of specific symptoms, rapid progression, and high malignancy [3, 4]. It is difficult to detect ovarian lesions in general detection, making early clinical diagnosis more difficult [5]. Cytoreductive surgery and adjuvant chemotherapy are common methods for the treatment of ovarian cancer, which are effective for more than 80% of patients with ovarian cancer and have achieved remarkable curative effects

Methods

Results

Discussion

Conclusion