Abstract
We examined the expression pattern of the small heat shock protein (sHSP) gene, DcHsp17.7, under various conditions. In non-stressed leaf tissue, DcHsp17.7 was absent or present at very low levels; however, exposure to an elevated temperature (40°C, 4 hours) led to an accumulation of this protein. Furthermore, after cessation of heat stress, the levels were sustained for two days, but diminished entirely thereafter. The persistence of DcHsp17.7 in post-heat-shocked leaves suggests that this sHSP may play a role during recovery after heat stress. Overnight heat stress (40°C, from 9 pm to 9 am) induced a higher level of DcHsp17.7, compared with that of plants exposed to heat stress during the day (40°C, from 9 am to 9 pm). These observations indicate that DcHsp17.7 gene expression is influenced by circadian rhythm. The levels of the DcHsp17.7 transcripts and their encoded protein increased after exposure to exogenous abscisic acid (ABA) and salicylic acid (SA), suggesting that these plant growth hormones may be involved in the expression of the DcHsp17.7 gene. Additionally, exposure to heavy metals, such as lead (Pb) and arsenic (As), led to increased levels of DcHsp17.7 transcripts and protein.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call