Abstract
PurposeTo describe cytokines, chemokines and growth factors profiles in patients undergoing cataract surgery with femtosecond laser pretreatment and investigate their relationships with the postoperative in vivo inflammation index.MethodsAqueous humor was collected from 22 eyes after femtosecond laser pretreatment and from 22 eyes at the beginning of routine cataract surgery. The levels of 45 inflammation-related mediators were measured using multiplex fluorescent bead-based immunoassays. Laser flare photometry was measured preoperatively and at 1 day, 7 days and 30 days postoperatively.ResultsCompared with the control group, the femtosecond laser treatment group showed significantly higher aqueous humor levels of fibroblast growth factor (FGF-2), tumor necrosis factor (TNF)-α, leukemia inhibitor factor (LIF), interleukin (IL)-1ra and IL-18, and significantly lower aqueous humor levels of IL-9, platelet-derived growth factor (PDGF)-BB, eotaxin and TNF-β. Postoperative aqueous flare was significantly greater in the manual cataract surgery group at 1 day (p<0.001), 7days (p<0.001) and 30 days (p = 0.002).No correlation was found between the analyzed mediators and the aqueous flare values.ConclusionsThe expression profiles of cytokines, chemokines and growth factors and the correlations of these profiles with the in vivo inflammatory indexes for patients undergoing cataract surgery with femtosecond laser pretreatment were identified. Our data indicate a disturbance of postoperative inflammation response after femtosecond laser treatment.
Highlights
In recent years, femtosecond laser technology has been introduced to the field of cataract surgery and offers many potential benefits
Postoperative aqueous flare was significantly greater in the manual cataract surgery group at 1 day (p
The sponsor of the study had no role in the design of the original study protocol, data collection, data analysis, data interpretation, writing of the report, or decision to submit the manuscript for publication
Summary
Femtosecond laser technology has been introduced to the field of cataract surgery and offers many potential benefits. Postoperative inflammation and the intraocular effect caused by surgical trauma and laser treatment are relatively unknown. It is not known whether the lens fragmentation of femtosecond laser-assisted cataract surgery, which leads to an increase in particulate matter in the anterior chamber, may exacerbates inflammation.[5,6] Previous studies have showed that postoperative aqueous flare was significantly greater with the manual cataract surgery group than with laser surgery.[7,8] Schultz et al reported that prostaglandins increase immediately after femtosecond laser treatment.[9] Postoperative inflammation is associated with the breakdown of the blood-aqueous barrier and is indicated by surgical trauma-induced cytokine production. Exploring a greater number of inflammation-relative mediators would provide broader insight into the inflammatory mechanisms involved in femtosecond laser pretreatment
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