Abstract

The human cytochrome P450 (CYP) superfamily comprises 57 genes, which encode enzymes important in the biotransformation of numerous endogenous and exogenous compounds. The aim of this study is to characterize the expression of all CYP genes in CD34(+) human cord blood hematopoietic stem and early progenitor cells (CBHSPCs), as a first step toward assessment of their biological functions in these cells. CD34(+) CBHSPCs were purified from umbilical cord blood via antibody affinity chromatography, using magnetically‐assisted chemical separation. Purity of CD34(+) cells was assessed using fluorescence‐activated cell sorting. RNA was isolated from purified CD34(+) cells for RNA‐PCR analysis, using primers specific for each of the 57 CYPs. Of the 57 CYPs, only 14 were detected by RNA‐PCR in CD34(+) CBHSPCs, including CYP2E1, CYP2U1, CYP2R1, CYP2S1, CYP2J2, CYP4F2, CYP20A1, and CYP27A1. CYP identification was confirmed by sequencing analysis of the detected, mRNA‐derived, PCR products. Preliminary quantitative analysis of CYP transcripts using real‐time RNA revealed preferential expression of multiple CYPs, such as CYP2R1, in CD34(+) CBHSPCs, compared to total lymphocytes or CD34(‐) cells from the cord blood. Additional studies on detection of CYP protein expression are underway.Grant Funding Source: Supported by State Scientific Key Projects for New Drug Research and Development(2011ZX09102‐010‐04)

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