Expression of CYP2W1 and its effect on proliferation and invasion of gastric cancer

Abstract

Objective To investigate the expression of CYP2W1 in gastric cancer and its effect on proliferation and invasion. Methods CYP2W1 protein expression in 326 gastric cancertissues and in the corresponding normal gastric mucosa was measured by immunohistochemstry. The expression of CYP2W1 mRNA in 10 randomly chosen gastric cancer tissues and its corresponding normal gastric mucosa was tested by semi-quantitative RT-PCR. 4 groups pairs of gastric cancer and normal gastric mucosa cell lines were constructed. CYP2W1 expression in each of the cell line was tested. The effect of CYP2W1 expression on the proliferation and invasion capacity of the gastric cancer cells was studied by MTT experiment and transwell cell experiment. Results Expression of CYP2W1 protein in the gastric cancer tissues is higher than that in normal gastric mucosa (26.7% vs.0, χ2=100.396, P< 0.05). CYP2W1 mRNA in the gastric cancer tissues is higher than that in normal gastric mucosa [(0.413±0.026) vs.(0.074±0.005), t=28.115, P<0.05]. CYP2W1 protein expression in the gastric cancer cell lines is higher than that in normal gastric mucosa cell lines[(0.481±0.024) vs.0, t=49.097, P<0.05]. The growth capacity of CYP2W1 positive gastric cancer cell is stronger than that of CYP2W1 negative cells (P<0.05), and CYP2W1 positive gastric cancer cells are also more of invasiveness, [(63±8) vs. (18±3), t=24.134, P<0.05]. Conclusions CYP2W1 is only expressed in the gastric cancer tissues, hence it is closely related to the growth multiplication, and invasiveness of gastric cancer cells. Key words: Stomach neoplasm; Cell proliferation; Neoplasm invasiveness