Abstract
Objective To investigate the effects of cyclinA2 and its inhibitor p21 on alveolar development in bronchopulmonary dysplasia(BPD) neonatal rats. Methods Eighty newborn rats were randomly divided into a model group(FiO2=80%-85%) and a control group(FiO2=21%). The degree of alveolar development was evaluated by radial alveolar count(RAC) and alveolar septal thickness.The distribution and expression of cyclinA2 and p21 were detected by immunohistochemistry and Western blot. Results The RAC value of the model group was lower than that of the control group from 3 days.The thickness of the alveolar septum was higher than that of the control group from 7 days(P 0.05); there was no correlation between RAC and p21 in the control group(r=-0.2929, P>0.05), and positively correlated with cyclinA2(r=0.8476, P<0.01). The alveolar septal thickness of the model group and the control group were both positively correlated with p21(r=0.4291, P<0.05; r=0.4447, P<0.05), and negatively correlated with cyclinA2(r=-0.6814, P<0.01; r=-0.7636, P<0.01). Conclusion The imbalance of cell cycle regulatory protein cyclinA2 and its inhibitor p21 expression in neonatal rats exposed to hyperoxia may be one of the related factors that interfere with the development of BPD alveoli. Key words: cyclinA2; p21; Alveolar dysfunction; Hyperoxia; Neonatal rats
Published Version
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