Expression of CXCL12/CXCR4, Topo II α and NF-κB in tissues of triple negative breast carcinoma

Abstract

Objective To explore the meaning of the expression of CXCL12/CXCR4, Topo Ⅱα, NF-κB in tissues of triple negative breast carcinoma (TNBC). Methods Fifty-three cases with TNBC were enrolled as TNBC group, and seventy-six cases with non-triple negative breast carcinoma (N-TNBC) as N-TNBC group.Immunohistochemical stainings were conducted with resection specimens, and proteins of CXCL12/CXCR4, Topo Ⅱ α, NF-κB were detected.Patients were followed up after surgery to record postoperative recurrence, metastasis and death.The death cases were recorded by censored data, and surviving cases were followed up for 3 years at least.Rates of recurrence and metastasis, mortality, and disease free survival time (DFS) were compared among different groups. Results Less than or equal to 50 years old, lymph node metastasis, invasive ductal carcinoma and Ⅲ stage were TNBC patients′ main features.Positive cells of CXCL12, CXCR4, Topo Ⅱ α, NF-κB were 66.04%, 84.91%, 69.81%, 79.25% respectively in TNBC group, and were 40.79%, 51.32%, 40.79%, 51.32% in N-TNBC group.Positive cells of CXCL12, CXCR4, Topo Ⅱ α, NF-κB in TNBC group were all higher than those in N-TNBC group (χ2=7.97, 15.51, 10.55, 10.43; P<0.05). Metastasis rate, mortality and DFS were 20.75%, 28.30%, (24.35±3.50)months respectively in TNBC group, and were 11.32%, 13.16%, (32.02±4.00)months in N-TNBC group.Metastasis rate, mortality in TNBC group were both greater than those in N-TNBC group (χ2=4.51, 4.58; P<0.05), yet DFS was less than that in N-TNBC group (t=5.29, P<0.05). Rates of recurrence and metastasis in positive expression groups of CXCL12, CXCR4, Topo Ⅱ α, NF-κB were 19.70%, 17.86%, 19.12%, 18.52%, and were 6.35%, 4.44%, 6.56%, 4.17% in negative expression groups, and they were all more significant than those in negative expression groups (χ2=5.02, 4.61, 4.43, 5.43; P<0.05). Mortality was 27.27%, 25.00%, 26.47%, 24.69% in positive expression groups, and was 11.11%, 8.89%, 11.48%, 10.42% in negative expression groups, and they were all bigger than those in negative expression groups (χ2=5.39, 4.87, 4.63, 3.93; P<0.05). DFS were (23.17±3.30)months, (24.50±3.21)months, (23.51±3.65)months, (25.36±3.65)months in positive expression groups, and were(32.25±3.55)months, (33.00±4.15)months, (35.00±5.10)months, (31.86±4.57)months in negative groups, and the differences between the two groups were statistically significant (t=5.37, 5.80, 6.21, 5.08; P<0.05). Conclusion There are high expression of CXCL12, CXCR4, Topo Ⅱ α, NF-κB in tissue of TNBC, with early recurrence and metastasis and poor prognosis, and it may be related with increasing tumor cells′ invasive ability and mediating specific metastasis of lymph node. Key words: Breast neoplasms; Prognosis; Neoplasm invasiveness