Abstract
Background: There is a growing body of research demonstrating expression of the renin-angiotensin system (RAS) by a putative embryonic stem cell (ESC)-like population within vascular anomalies. This study investigated the expression of components of the RAS in relation to the putative ESC-like population within pyogenic granuloma (PG) that we have recently reported.Methods: PG samples from 14 patients were analyzed for the expression of components of the RAS: pro-renin receptor (PRR), angiotensin converting enzyme (ACE), angiotensin II receptor 1 (ATIIR1) and angiotensin II receptor 2 (ATIIR2), using 3,3-diaminobenzidine (DAB) immunohistochemical (IHC) staining. Immunofluorescence (IF) IHC staining was performed to localize these proteins on four of the PG samples. RT-qPCR was performed on two snap-frozen PG samples. Western blotting (WB) was performed on one snap-frozen PG sample and two PG-derived primary cell lines.Results: DAB IHC staining demonstrated the expression of ACE, PRR, ATIIR1, and ATIIR2 in all 14 PG tissue samples. RT-qPCR analysis confirmed abundant mRNA transcripts for PRR, ACE, AIITR1 and ATIIR2, relative to the housekeeping gene. WB confirmed the presence of PRR, ATIIR1, and ACE in the PG tissue sample, and PRR and ATIIR1, in the PG-derived primary cell lines. IF IHC staining demonstrated the expression of PRR, ACE, ATIIR1 on the primitive population that expressed NANOG and SOX2 on the ERG+ endothelium of the microvessels within PG.Conclusion: We have demonstrated the expression of PRR, ACE, and ATIIR1 by the putative the ESC-like population within PG.
Highlights
Pyogenic granuloma (PG) is a relatively common benign vascular tumor affecting the skin and mucosa
PG tissue samples from four female and 10 male patients with an average age of 21.3 years were sourced from the Gillies McIndoe Research Institute Tissue Bank and used for this study which was approved by the Central Regional Health and Disability Ethics Committee (Ref. 13/CEN/130)
DAB IHC staining demonstrated the expression of pro-renin receptor (PRR) (Figure 1A, brown) on the endothelium of the microvessels and cells within the perivascular tissues, while angiotensin converting enzyme (ACE) (Figure 1B, brown) was expressed on the endothelium of the microvessels, in PG
Summary
Pyogenic granuloma (PG) is a relatively common benign vascular tumor affecting the skin and mucosa. PG arises spontaneously, in previously injured tissues or within vascular malformations [1] and has been associated with certain medications, such as chemotherapeutic agents [2]. Renin-Angiotensin System in Pyogenic Granuloma for 0.5% of skin nodules in the pediatric population, most commonly in the first five years of life [1]. There is a growing body of research demonstrating expression of the renin-angiotensin system (RAS) by a putative embryonic stem cell (ESC)-like population within vascular anomalies. This study investigated the expression of components of the RAS in relation to the putative ESC-like population within pyogenic granuloma (PG) that we have recently reported
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