Abstract
Integration of H2-uptake (hup) gene cosmid pHU52 into the chromosomal DNA, conferred H2- uptake activity on the Hup- Cicer-Rhizobium strain G36–84 in the free-living state and in nodules. In five transconjugants (G36–84:: Tn5:: pHU52) derepressed for hup gene expression, the specific Hup activity ranged from 158 to 256 nmole H2 hr-1 mg-1 protein which was 42 to 64% lower than the activity obtained in transconjugant with pHU52 as an episome. Integration of the cosmid significantly improved the relative efficiency of symbiotic N2-fixation by imparting H2-recycling capability to Hup-Cicer-Rhizobium. Demonstration of Hup activity in the nodules of field grown chickpea plants suggests that the integrated hup genes are stably maintained in natural environment
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