Abstract
Chemokine and chemokine receptor expression by tumor cells contributes to tumor growth and angiogenesis and thus these factors may be considered as tumor markers. Here we aimed to characterize cells directly extracted from glioma, meningioma, and secondary brain tumors as well as non-tumoral cells in vitro. Cells were isolated from brain tissues using 0.2% collagenase and characterized by flow cytometry. Expression of SDF-1, CXCR4, CXCR7, RANTES, CCR5, MCP-1 and IP-10 was defined using flow cytometry and qRT-PCR methods. Brain tissue isolated cells were observed as spindle-shaped cell populations. No significant differences were observed for expression of SDF-1, CXCR4, CXCR7, RANTES, CCR5, and IP-10 transcripts. However, the expression of CXCR4 was approximately 13-fold and 110-fold higher than its counterpart, CXCR7, in meningioma and glioma cells, respectively. CXCR7 was not detectable in secondary tumors but CXCR4 was expressed. In non tumoral cells, CXCR7 had 1.3-fold higher mRNA expression than CXCR4. Flow cytometry analyses of RANTES, MCP- 1, IP-10, CCR5 and CXCR4 expression showed no significant difference between low and high grade gliomas. Differential expression of CXCR4 and CXCR7 in brain tumors derived cells compared to non-tumoral samples may have crucial impacts on therapeutic interventions targeting the SDF-1/CXCR4/CXCR7 axis.
Highlights
IntroductionChemokines and chemokine receptors are constitutively expressed by microglial cells, astrocytes, and neurons and play crucial roles in central nervous system (CNS) from development to neoplastic process (Bajetto et al, 2001)
Brain tumor is one of the leading causes of cancer related death worldwide
SDF-1 showed the statistically significant higher expression in both meningioma and glioma cells when compared to its receptors, CXCR4 (p
Summary
Chemokines and chemokine receptors are constitutively expressed by microglial cells, astrocytes, and neurons and play crucial roles in central nervous system (CNS) from development to neoplastic process (Bajetto et al, 2001). Chemokines and their receptors are involved in many pathological processes including inflammation, angiogenesis, and tumor cell growth and dissemination (Bajetto et al, 2001; Bian et al, 2007). SDF-1 may act as a stimulant for proliferation and survival of glioblastoma multiform, medulablstoma cell lines and xenografted tumors (Bajetto et al, 2007)
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