Abstract
ABSTRACT Gene expression of CDKN1A, CDKN1B, and TP53, and immunostaining of p21, p27 and p53 were evaluated to verify the role of these cell cycle inhibitors in canine prostates with proliferative inflammatory atrophy-PIA and prostatic carcinoma-PC. Seventy samples, 15 normal, 30PIA and 25PC. Regarding number of p27 and p53 labeled cells, difference between normal and PIA and PC was observed, as well as between PIA and PC for p53. Immunostaining intensities of p21, p27 and p53 were different when comparing normal tissues to PIA and PC. Sixteen cDNA of canine prostatic FFPE tissue were subjected to RT-PCR and RT-qPCR, four normal, three PIA, and nine PC. CDKN1A mRNA was detected in four PC by RT-PCR, and it was overexpressed when compared to normal by RT-qPCR, in one PIA and six PC. CDKN1B mRNA was detected in three PC by RT-PCR and it was overexpressed in three PC and decreased in one PC. TP53 mRNA was overexpressed in one PIA and three PC. In conclusion, when overexpressed in canine prostate with premalignant and malignant, p21 and p27 play a role controlling cell proliferation, working as a protective factor in the evolution of PIA to PC, and in the PC development, even in the presence of altered p53.
Highlights
The canine prostate has similarities with the human gland regarding the occurrence of benign and malignant diseases such as benign prostatic hyperplasia (BPH) and prostatic carcinoma (PC) (Leroy and Northrup 2009)
The number of p27 stained cells and immunostaining intensity was different between normal prostatic tissue and Proliferative inflammatory atrophy (PIA), and between normal tissue and PC, but it did not differ when comparing PIA with PC
The number of p53 stained cells was different between normal prostatic tissue and tissues with PIA and PC, as well as between prostates with PIA and PC
Summary
The canine prostate has similarities with the human gland regarding the occurrence of benign and malignant diseases such as benign prostatic hyperplasia (BPH) and prostatic carcinoma (PC) (Leroy and Northrup 2009). Proliferative inflammatory atrophy (PIA) is a human prostate lesion with premalignant potential and involvement in carcinogenesis of PC (Wang et al, 2009). Rodrigues et al, (2010) have mentioned PIA in canine prostate and Toledo et al (2010) described its histological aspects. The major step in malignant transformation in tumors is the loss of cell cycle control. Knowing how regulators work is a key step to understand malignant transformations that occur in tumor types (Sanchez and Dynlacht, 2005)
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Arquivo Brasileiro de Medicina Veterinária e Zootecnia
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
