Abstract
Objective:To investigate the expression of aquaporin 5(AQP5)and MUC5AC in lung of bronchial asthmatic murine model and the regulatory effect of dexamethasone on the expression.Methods:Forty-eight female C57BL/6 mice were evenly randomized into control group,asthma group and dexamethasone group.The pathological changes of the lungs and the wet/dry weight ratios of the lungs were determined in mice of each group.The expression of AQP5 and MUC5AC mRNA in the lung was determined by real-time poly-merase chain reaction(real-time PCR);the AQP5 protein was examined by Western blotting assay;the distribution of AQP5 protein in the pulmonary tissue was studied immunohistochemically;and the content of MUC5AC in the bronchial alveolar lavage fluid(BALF)was determined by ELISA.Results:Compared with the control group,the pulmonary expression of AQP5 in asthma group was obviously decreased(mRNA by[42.5±3.6]%,protein by [64.3±8.2]%),while the expression of MUC5AC was increased(mRNA by[93.3±8.1]%,protein by[98.3%±7.2]%).Dexamethasone significantly decreased the pulmonary expression of AQP5 and MUC5AC compared with the asthma group(P0.05).Conclusion:The decreased pulmonary expression of AQP5 in asthmatic mice might be related to the elevation of MUC5AC expression in the airway;dexamethasone can decrease AQP5 expression and therefore alleviate the overproduction of airway mucus,pulmonary inflammation and lung edema.
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